Recently, the Chengdu Institute of Biology, Sichuan Province, Chinese Academy of Sciences has developed a successful electrospray ionization mass spectrometer based on electrically conductive nanomaterials and its method for electrospray mass spectrometry. It has also obtained the invention patent of the State Intellectual Property Office.
The Chengdu Institute of Biology, Chinese Academy of Sciences, was established in 1958. It is a research institute directly under the Chinese Academy of Sciences under the first-level discipline. Chengdu Institute of Biology Public Experimental Technology Center has a variety of common experimental equipment, with 600MHz nuclear magnetic, high-resolution mass spectrometry, amino acid automatic Analytical instruments, multi-functional microscopes and other types of advanced instruments and equipment. At present, Chengdu Institute of Biology has achieved more than 300 scientific and technological achievements, among which more than 100 awards have been awarded by provincial and ministerial level scientific and technological achievements. Historically, Chengdu Institute of Biology has been working on electrospray ions. Technology has a deep research.
Electrospray ionization technology was introduced in the 1970s. It has a soft ionization characteristic that is not susceptible to fragmentation of compounds. It is the most widely used ionization method in the field of mass spectrometry. However, traditional techniques such as the inability to directly analyze high-salt-containing organisms The disadvantages of the sample require prior desalting of high-salt samples and the use of liquid chromatography with buffered salts.
In 2017, the research project of the development of scientific research equipment of the Chinese Academy of Sciences under the chairmanship of the Chengdu Institute of Biology received the final acceptance of the development of the electrospray ionization source for the biological mass spectrometer probe. The Chengdu Institute of Biology has continuously optimized the control method and sample loading method. The high-voltage switch-on method and ion transmission method make it resistant to high-voltage interference, salt resistance and matrix interference. Based on this, the liquid-phase interface has been further developed, enabling the use of high-salt buffer solvents for the ion source. The combination of liquid chromatography, and has successfully developed the equipment.
During the R&D process, the Chengdu Institute of Biology encountered new problems. The electrospray ionization process is usually performed in polar solvents. This ionization technique is suitable for ionization analysis of medium and high polarity systems. However, many compounds only It is soluble in low polarity solvents, and this sample is difficult to ionize by electrospray, which makes ESI-MS limited in the analysis of low polarity solvent system and partial organic reaction mechanism.
In response to the difficulties encountered, Chengdu Institute of Biological Science and Technology has proposed an electrospray mass spectrometer based on conductive nanomaterials and a method for electrospray mass spectrometry analysis. In addition to ionizing compounds dissolved in polar solvents, the method can also It is preferable to ionize compounds dissolved in a low-polarity solvent and satisfy the mass spectrometry requirements for polar and low-polarity systems, and the method is simple and inexpensive.