According to the U.S. technology website Ars Technica, the 4 bases of DNA (A, C, G, T) can change the currents and voltages on both sides of the nanopore in different ways and by measuring the change in current and voltage, respectively For single-stranded DNA sequencing.
Six years later, researchers are finding new uses for the sequencer by focusing on the properties of these protein nanopores. In a recent paper published in the journal Nature Biotechnology, Researchers at the university introduced the long read that the sequencer provided and how it can be used to sequence the human genome that was previously resistant to characterization.The device can also be used to decide on two reads from another gene Chromosomes, which map the epigenetically controlled regions of the entire genome.
The new use of the sequencer was developed by Nick Loman, a professor at the Institute of Microbiology and Infectious Diseases at the University of Birmingham, and Josh Quick, a PhD student in the University of Birmingham, where Quick plays an important role in developing the reader-in-law method.
Although the sequencer is still error prone, it outperforms the more accurate sequencer when errors occur, especially when reading DNA with more than 200 bases. When the DNA is repeated, the other Software programs, and when a highly accurate device is used with a small device from Oxford Nanopore, it offers a compromise between sequences and shows how the sequence can form a larger part.
Using different software solutions and voltage data interpretation data, the study explored several ways to generate the best nanopore sequencing.The researchers performed multiple reads and plots in a variety of ways, ultimately achieving 99.44% accuracy .
Ars Technica reports that because we inherit the two duplicated chromosomes from our parents, respectively, though the underlying DNA is the same despite the different versions, this means that the short, long DNA does not determine the difference between the two, The long readout provided by the nanopore was of the utmost importance, and during the course of the experiment, researchers found that its reading length was as high as 882,000 bases, which was not possible with the original genome sequencing project and the researchers predicted that The new application will help fill all vacancies in the original genome project.
Researchers did encounter some challenges during the study as they found that the generic file specification used to save DNA data could not handle long sequences that led to failure of the analysis software.Of course, if software could be updated to include this functionality, Then the possibility of future applications is endless.
Loman said: 'Even a year ago, it was virtually impossible to sequence the entire human genome, but with the recent advances and innovations in nanopore technology, we have now been able to sequence very long genomic segments He also compared this sequencing method to a puzzle.
"One of the most important findings in this study is that even after completing a human genome reference or thinking it has been done for some time, it still contains many deficiencies and the new method we developed developed using nanopore sequencing Out of long reading length, thus shortening the sequence of some gaps.
Compile: Susan Hong