| Researchers at Japan's Saga University and Nagasaki University conducted stent-free tracheal regeneration using the Cyfuse Biomedical Regenova 3D bio-printer. Nine rats were successfully treated with tracheal transplantation.  Stability is the key when trying to bioenginear new tracheal or airway organs.Read so: If a transplanted, biological 3D-printed ear crashes, the recipient of the transplant is a problem, but not a life-threatening one, but If a 3D-printed trachea is paralyzed, patients can be in trouble because they lose their ability to breathe. Scientists have different opinions on how to ensure tracheal stability: Some bioengineers think stents are the best way to make artificial airway organs, but this approach can present problems and limitations, which is why it is led by Koichi Nakayama of the Saga University , A group of researchers who conducted a three-dimensional study of rat liver tissue at Zhongshan Laboratory who used a 3D bio-printer to make a stent-free artificial trachea, said that their 3D-printed trachea is strong enough to prevent collapse and test The structure of the mouse to prove this. In collaboration with many researchers at Nagasaki University, Nakayama hopes to conduct a study to develop a new scaffolding-free way to create artificial trachea using some of the most advanced 3D bio-printing techniques in which researchers Scaffold-free tracheal-like grafts produced from isolated cells were made in an inbred animal model. Although Nakayama has admitted that the research budget is low, the researchers have an impressive bio-printing machine, Cyfuse Biomedical's Regenova bioprinter. Regenova is unique in using the Kenzan method, which is a bit weird, Disperse spheroids of cell clusters onto sharp spikes to maintain their position, which may be the most advanced kebab in the world! Japanese researchers delivered Regenova to F344 mice and chondrocytes and mesenchymal stem cells were harvested for rat lung microvascular endothelial cells, respectively, and researchers were then able to use the Regenova 3D bioprinter to 3D print tiny Artificial trachea allows the structure to mature in the bioreactor to ensure cell growth. In this incredible bioprinting process, Cyfuse Biomedical printers place the spheroids in a 9 x 9-pin array of 3.2 mm length on each side.The outer diameter of each stainless steel needle is 0.17 mm, 0.4 mm The cell spheres were aspirated by a robot-controlled 96-well robot nozzle No. 25. A total of 384 spheroids were used to create the tubular 3D-printed trachea. Once fully matured, a 3D printed unsupported trachea was grafted into F344 rats as tracheal grafts with each animal under general anesthesia, and then, once implanted, the mechanical strength of the artificial trachea was measured, histology and immunohistochemistry Chemical examination. A total of 9 rats were used in the experiment, which was examined carefully for 23 days each day after transplanting the bio-printed trachea.During the examination, the researchers found that the 3D-printed trachea was strong enough to be grafted with a silica gel stent To prevent collapsing of the artificial trachea and supporting the graft until there is sufficient supply of blood Histologically, chondrogenesis and angiogenesis are observed. Although two lab rats experienced 'wheezing' due to stranded tracheal secretions, these researchers said their bio-printed trachea was a tremendous success. What happened? 3D printing appears to be creating an artificial Is it an effective tool for tracheal replacement? Can it do the same for humans? Time will tell. Source: China 3D printing network |
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