Tianjin Inspection and Quarantine Animal Quarantine Laboratory has established a fast and easy, highly specific and highly sensitive fluorescent PCR detection system, which can be quickly, accurately, specifically, safely and simply tested in the sample within 3 ~ 4 hours ASFVCP530R gene.
According to the feedback from the State Intellectual Property Office, the "fluorescent PCR detection reagent of CP530R gene of African swine fever and its preparation method and application" declared by Animal Inspection and Quarantine Laboratory of Tianjin Inspection and Quarantine Bureau have officially obtained the invention patent authorization certificate.
According to the introduction, the invention discloses a fluorescence PCR detection reagent of the CP 530R gene of African swine fever virus and a preparation method and application thereof, and a set of specific primer, Taqman probe and positive control are designed and synthesized. By using the primer and the probe A fast, simple, specific and sensitive fluorescent PCR detection system was established to rapidly, accurately, specifically, safely and simply detect the ASFVCP530R gene from the tested samples in 3 ~ 4 hours. Detection of the CPV30R gene from various samples of domestic pigs, wild boars and soft ticks.
African Swine Fever (ASF) is an acute, hot, highly contagious disease of swine caused by the African Swine Fever Virus (ASFV), which spread from Africa to Europe and the United States beginning in the 1960s and 1970s. In 2007, Georgia , Armenia, Azerbaijan and Russia all have great epidemic, which pose a potentially huge threat to the continuous and stable development of China's pig industry.Therefore, the establishment of rapid, sensitive and specific detection methods can provide powerful technologies for the prevention and control of ASFV means.
